![]() For sake of simplicity, let us think about the Super-Resolution task. In conditional GANs, we are starting from label, image, or etc. However, most of the times we are trying to add condition to the GANs. GANs are able to learn such distribution in an indirect fashion. Some times our aim is producing new samples by learning the underlying data distribution. In general, generative adversarial network is able to generate super realistic images by starting from the noise vector. In advance, I will appreciate your time and thanks for that. I am looking for your opinions and welcome to put any thoughts. Does this mean what grew on the chlora plate was one of those mutated CCDB genes that acquired chlora resistance? I checked the gateway manual and it said (see attached picture 2) that sometimes the CCDB gene gets mutated and becomes chlora-resistant. Not sure why? Shouldn't it not grow on chlora? Sequencing showed that the gene was indeed in the plasmid. I transformed one of the maxipreps I had on an amp plate and did the same with a chlora plate and they both grew colonies. Therefore, true positive clones would ONLY grow on ampicillin plates. Thus, this eliminates the positions from attr1 through attr2.removing the gene encoding chloramphenicol resistance and inserting your cDNA there. From my understanding, during the LR reaction.your entry clone containing the cDNA of interest gets integrated into the destination vector (pDEST15) which happens through a process that involves flanking the two attr sites on pDEST15. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I am having a few doubts about gateway cloning. Photo 2 is the characteristic GFP expression I was expecting, from the original GFP vector. Photo 1 is a plant infiltrated with my new vector. Does anybody have any ideas as to why the vector I made expresses GFP that is paler and weaker instead of the normal bright green GFP fluorescence? I attached two photos for reference. The original vector that I got GFP from before I cloned it into my new vector produces the characteristic bright green fluorescence, as well. This is in comparison to what I was expecting, the characteristic bright green fluorescence usually seen when using a GFP-expressing viral vector. As in, the viral vector is definitely replicating and moving systemically, however, the GFP fluorescence is a pale, light green. However, after infiltrating my new vector into benthamiana, the GFP expression is very pale and weak. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme cloning to clone the GFP into the new viral vector I made. I made a plant viral vector expressing GFP. ![]()
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